This SOP sets the safety and procedural specifications for making a 1M stock solution of calcium chloride. Calcium Chloride (20-25gr) Water (50 ml) Two types of Ziplock Bag. Grow plate overnight at 37°C. Materials 1L stock of 1M CaCl 2 (makes ~3000 kits) 1. Calcium Chloride it's really easy to found in a store, it's use for Dehumidifiers the home or to put on the frozen Sidewalk for melt the ice! Objective . For example, when making up a 30% solution from DOWFLAKE™ Xtra 83-87% Calcium Chloride Flakes, the solution temperature may be expected to increase approximately (30 x 2.8°F) = 84°F. Silica Gel Method Step 1 Poke seven or eight holes in the lid of a clean, disposable plastic container with the pointed end of a pair of scissors or a sharp knife CALCIUM CHLORIDE: The authors make no claims of the accuracy of the information contained herein; and these suggested doses are not a substitute for clinical judgment. Solid CaCl 2. Newer, more in depth text on zymurgy will support the use of calcium chloride. 2. onto an LB plate (no antibiotics since these cells do not have a plasmid in them).Work sterile. Make a homemade version of this product inexpensively by using the correct ingredients. Calcium chloride is great for altering a water profile to fit the style of beer. The small quantities used in beer and cheese making should be less of a health concern than the air most of us breath. For calcium chloride, you can add hydrochloric (muriatic) acid to calcium carbonate (limestone or marble chips) until the latter all dissolve, and then dry it by boiling or evaporation. For use by biotechnology students at the ScienceBridge tech sites when making calcium chloride solution for the ScienceBridge RNAi kits. Damp Rid contains calcium chloride crystals which absorbs excess moisture in the air naturally. You can reuse the liquid for this last method or for kill the rogues. Making Calcium Competent Cells Day 1 1. That being said, this is only my opinion… Streak out frozen glycerol stock of bacterial cells (Top10, DH5α, etc.) A spreadsheet tool is available to determine the amount of dry calcium chloride to mix with water to achieve solutions of various concentrations.